Prenatal chromosome analysis | Classical cytogenetics

Test Details Detects aneuploidy, rearrangements, deletions, inversions
Methodology Chromosome analysis / karyotype
Performed Monday – Saturday
Turnaround 14 days
Specimen Requirements Amniotic fluid (AF), chorionic villi sampling (CVS), percutaneous umbilical blood sampling (PUBS)
Sample Collection AF: Discard first 2mL of amniotic fluid. Collect 15-25mL in a sterile syringe. Use sterile technique to transfer to 15mL sterile screw-top plastic conical tubes for transport. Do not centrifuge. CVS: Collect 15-40mg of budding villi in 50mL screw-top plastic conical tubes in sterile transport media (provided by cytogenetics lab). Do not centrifuge. PUBS: Collect 1-3mL in sterile, 15mL sodium heparin (green-top) Vacutainer tube. Collect under ultrasound guidance. Invert tube immediately upon completion of blood collection to prevent formation of clots.
Storage/Transport Conditions Room temperature
Unacceptable Conditions Will not be accepted after three days
Special Instructions Refrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving: Uniquely identify twins/multiples
CPT Code(s) 88291×1, 88235×1, 88269×1, 88280×1